Telomere Fish Kit

Association Of telomere Instability With Senescence Of ...
In pigs. Telomere quantitative fluorescence in situ hybridization (Q-FISH), however, can reveal status of individual telomeres in fewer cells, in addition to quantifying relative telomere lengths, and has been commonly used for study of telomere function of mouse and human cells. We attempted to investigate telomere characteristics of ... Read Document

Chromosome Number And telomere Sequence Mapping Of The ...
Introduce the FISH technique in Holothuroidea research. To achieve this, we performed FISH with a telomere probe for the A. japonicus chromosome, because a telomere is composed of a simple tandem repeat sequence and it is easier to use this sequence for FISH on the chromosome rather than on a single-copy sequence. ... Retrieve Content

Telomere Erosion In Memory T Cells Induced By Telomerase ...
Cell Permeabilisation Kit; Caltag Laboratories) before staining for CD3, CD4, IL-2, and IFN-. Measurement of Telomere Length by Flow Cytometric Detection of Fluorescence In Situ Hybridization (Flow-FISH). Telomere length of CD4 T cells was measured using a modified two-color flow-FISH protocol (21). The cells were stained with CD4-biotin ... View Doc

Genetic Engineering - Wikipedia
Genetic engineering, also called genetic modification or genetic manipulation, is the direct manipulation of an organism's genes using biotechnology.It is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. ... Read Article

Telomerase Is Required To Slow telomere Shortening And Extend ...
Fluorescent in situ hybridization Telomeres were detected by FISH using a FITC-conjugated peptide nucleic acid telomeric probe ((CCCTAA) 3 , Applied Biosystems), as described ... Doc Viewer

Dissociation Of Telomerase Activity And telomere Length ...
To assess telomere length in cells, flow-FISH analysis of cells hybridized with a telomere-specific FITC-conjugated peptide nucleic acid probe was performed as described (29, 30). Mean telomere length was calculated as described (30) by using cow thymocytes as an internal standard. Samples were performed in duplicate, and SD is indicated by ... Read Full Source

Application Of Primed In SituDNA Synthesis (PRINS) With ...
Technique with telomere human commercial kit to telomere sequences identification. This commercial kit may be use to visualization of interstitial telomeric signal in pig genome. ... Retrieve Full Source

The Arabidopsis telomere Sequence Is Highly Abundant In The ...
Fluorescence in situ hybridization (FISH) was carried in order to determine the chromosomal localization of the telomere repeat (TTTAGGG)5 in the P. acutifolius accessions NI 576, NI 562, as well as in P. vulgaris and P. coccineus. ... Retrieve Document

PROFESSOR HOLGER PUCHTA (Orcid ID - KIT
Telomere FISH signals which indicates a certain degree of telomere clustering since the expected number of telomeres based on a chromosome complement of 2n = 38 would be 76 in 2C nuclei (Supplementary File 1). ... Access Full Source

Powtarzalne aberracje chromosomowe są wykrywane za pomocą klasycznego badania cytogenetycznego i uzupełniająco fluorescencyjną hybrydyzacją in situ (FISH). Krew obwodowa oraz szpik kostny są poddawane badaniom molekularnym. RNA i DNA są ekstrahowane, a komórki są zamrażane. ... Read Article

Study Of Telomere Dysfunction In TP53 Mutant LoVo Cell Lines ...
(TRF) assay, the 3D quantitative telomere fluorescence in situ hybridization (FISH) on nuclei, quantitative telomere FISH on metaphases, and spectral karyotype, respectively. TRF assay evaluates the average length of telomeres and is based on digestion of genomic DNA by restriction enzymes that do not cut telomeric sequences. ... Access This Document

Telomere Lengths In Human Oocytes, Cleavage Stage Embryos And ...
Telomeric PNA probe (DAKO, Denmark; Telomere PNA FISH kit/ FITC, K5325) with modifications. To provide a quantitative control enabling comparison between slides prepared on different occasions, a suspension of L-5178Y-S cells, which have known telomere length, was added to the slides where the oocytes or embryos were fixed. All cells ... Retrieve Full Source

Space Ambient For Positive Vibrations - YouTube
Contemplative Side of Ambient Electronica. Music for Positivity and Inward Reflection. ... View Video

Q-FISH TELOMERE LENGTH MEASSUREMENT PROTOCOL
Q-FISH TELOMERE LENGTH MEASSUREMENT PROTOCOL . A) Day I: METAPHASE OBTENTION . 1. Add Colcemid (100µl stock 100X to 10ml of complete media) to cells in culture. Incubate 2-4h culture cells at 37C (2h=lymphocytes, immortalized cells; ... Fetch Full Source

Telomere Amount And Length Assay - Link.springer.com
Removal Kit (QIAGEN Inc, Valencia, CA). Telomere Amount and Length Assay (TALA) The hybridization of telomere DNA fragments with the telomere probe was performed using solution hybridization (6), with the following modifications. Genomic DNA (2.5 g) was digested with restriction enzymes, i.e., 10 units each of ... Fetch Content

Telomere Length Differences Upon Keratinization And Its ...
Cells. In addition, fluorescence in situ hybridization (FISH) study showed that columnar groups of keratinocytes with statistically longer telomere in each nucleus than the other epidermal cells in the vicinity occupied occasionally between the tip of dermal papilla and the bottom of rete ridge. ... Read Here

Nanoscale Properties Of Human Telomeres Measured With A Dual ...
Situ fluorescence hybridization (FISH) kit (Dako, Denmark), replacing their probe with our own. It should be noted our FISH protocol (and most FISH protocols in general) uses proteases and ribonucleases to strip the cell of all components other than the DNA contained in the nucleus. Thus, the images shown are solely from DNA. ... Doc Viewer

Tpp1/Acd Maintains Genomic Stability Through A Complex Role ...
Sequences, demonstrating a role in telomere protection in vivo. Surprisingly, our studies also reveal fusions and radial structures lacking internal telomere sequences, which are not anticipated from a simple deficiency in telomere protection. Employing spectral karyotyping and telomere FISH in a combined approach, we have ... Fetch Document

Myc - Wikipedia
Myc is a family of regulator genes and proto-oncogenes that code for transcription factors. The Myc family consists of three related human genes: c-myc, l-myc, and n-myc. c-myc (also sometimes referred to as MYC) was the first gene to be discovered in this family, due to homology with the viral gene v-myc. ... Read Article

METHODOLOGY Open Access A Quantitative PCR Method For ...
Sation of digested DNA with telomere sequence probes, (ii) Flow-FISH cytometry of cells following hybridisation with fluorescent peptide nucleic acid (PNA) probes, (iii) quantitative fluorescence in situ hybridisation (FISH) with fluorescent telomere PNA probes and (iv) qPCR assay. With the exception of the TRF assay all other meth- ... Access This Document

Cell Reports Supplemental Information PGC 1 Modulates ...
Telomere Length Assay by Measurements of Telomere Restriction Fragments (TRF) To measure telomere length, we performed the analysis of terminal restriction fragment (TRF) length by Southern blot (Prowse and Greider, 1995). TRF length was assessed by the TeloTAGGG telomere length assay kit (Roche) according to the manufacturer's instructions. ... Read Content

Telomere Transcripts Target Telomerase In Human Cancer Cells
Of TERRA with telomere maintenance and cancer is a vital research topic and described in several reviews [10–15]. TERRA may be a promising biomarker and potential tool in anti-cancer therapy [12,13]. ... View Document